Academics

• Ph.D (Molecular Biology), CEMB, University of the Punjab, Lahore
• M.Sc (Microbiology), CVS, Agriculture University, Faisalabad
• B.Sc (Biosciences), FC collage, Lahore

Dr. Ahmad Usman Zafar is working as an Associate Professor, Department of Biotechnology at the Qarshi University, Lahore. He had 34 years Teaching & Research experience at Centre of Excellence in Molecular Biology (CEMB), Punjab University, Lahore. He established state of the art functional labs for upstream and downstream processing of recombinant human proteins at CEMB. He had R&D training on Hormonal Studies at School of Medicine, University of Pennsylvania, Philadelphia, USA; Laparoscopic embryo transfer at Malaysian Agriculture Research and Development Institute, (MARDI) K. L. Malaysia; Recombinant human biopharmaceuticals and Bioassays at International Centre of Genetic Engineering and Biotechnology (ICGBE), Italy. Also visited as scientist exchange program for Biopesticide studies at Nanjing Agriculture University, Nanjing, China.

Work Experience (Summary)

Teaching Experience

• 34 years Teaching experience (BS/ M.Phil / Ph.D Classes)
• Supervised 40 M.Phil/Ph.D thesis research works of students as supervisor and/or co-supervisor.

Pharmaceutical Consultancy to:

• BF Biosciencs, Lahore
• Pharmedic Pharma, Lahore
• Getz Pharma, Karachi
• Sami Pharma, Karachi
• Mecter Pharma, Karachi
• NTP Gelatin Pvt. Ltd., Lahore
• Livewell Capsules Pvt. Ltd. Lahore

Research papers / Conferences

Foreign Trainings / Workshops / Seminars

2019 Talk on “Development of Indigenous technology for the commercial production of recombinant human proteins for therapeutic use” at 2nd meeting of Cancer Research Group, University of the Punjab, Lahore.

2019 Talk on “Biowaste management system and Incineration” at workshop Training, organized by Health Security Partners, Gull’s & CEMB on 21-22 Feb. 2019.  

2019 Participate “A short course on Cancer Biology” organized by Cancer Biology Lab, MMG & Hormone Lab, on 12th-14th Feb. 2019 at University of the Punjab, Lahore.

2018 Participate as Organizer for 3rd International symposium on “Advances of Molecular Biology in Plants and Health Sciences” at CEMB, Lahore, on 19-21 Dec, 2018.

2018 Participate Workshop on  “Dual use Research of Concern (DURC)”  organized by Health Security Partners at CEMB on 16-18 July, 2018.

2018 Participate “Institutional Biosafety Committee” Training Workshop at COMSTECH, Islamabad, organized by Fogarty, NIH and Pakistan Biological Association (PBSA) on 15-16 Feb., 2018.

2017 Participate as Organizer & Poster presentation, International symposium on “Advances of Molecular Biology in Plants and Health Sciences” at CEMB, Lahore, on 21-23 November, 2017.

2016 Invited speaker at the one day workshop on “A day with Biosafety” jointly organized by Congress of Molecular Biology and CEMB on Oct. 25, 2016.

2016 Participate “Cancer Awareness Seminar” held at CEMB on Feb.26, 2016, with the collaboration of Cancer Care Hospital & Research Centre.

2015 Participate and Organizer of “Advances in Molecular Biology of Plants and Health Sciences” held at CEMB, Lahore, on 29-31 December, 2015.

2015 Participate hand on training workshop on “Next Generation Sequencing Data Analysis” held at CEMB on Oct. 14-16,2015.

2013 Workshop on “Present and Future needs of Interferon for treatment of Hepatitis” at Centre of Excellence in Molecular Biology, Lahore on Feb.1, 2013.

2011 Resource person in the training course on “Advances in Applications of Molecular Biology” held at CEMB, University of the Punjab, Lahore, Dec. 19-23, 2011.

2010 Participation in the international symposium on “Biotechnology applications in new emerging fields” at Allama Iqbal Medical College, Lahore on December 21-23,2010.  

2009 Presentation in a symposium / workshop on “Future trends in molecular biological research and its applications in Agriculture & Health” at CEMB, Lahore on March 25-28, 2009.

2009 Training on “Internal Auditing, Corrective & Preventing Actions” by Quality Management Consultants conducting on March 12,2009.

2008 Presentation on “Interferon for Hapetitis” 18^th meeting of the standing committee at LCCI, Lahore, 28^th Oct. 2008.

2008 Workshop on Approval Process for Biologicals held on National Bioforum 2008 at Centre for Molecular Biology, Lahore, March 24-28, 2008.

2007 Participated in a workshop on “Clinical Research and its Industrial Implications” organized by National Core Group in Life Sciences-HEC and Bahria University, Islamabad on 07-08, 2007.

2007 National training seminar on “Biosafety and Biosecurity Initiatives”jointly organized by PARC, Sandia National Laboratories and US Civilian Research & Development Foundation, at Marriott Hotel, Islamabad, June 18-19, 2007.

2007 Certificate of participation and presentation on “Design and development of recombinant vaccines” in an International workshop on Vaccine Production and Quality Control, organized by National Core Group in life Sciences HEC and Comstech at Comstech Secretariat, Islamabad, May 28^th to 29^th,2007.

2006 Certificate of participation in the workshop on ” Biochemical Engineering and Fermenter Applications” held at IBB, University of the Punjab, Lahore on August 19,2006 with the collaboration of Bioengineering Switzerland..

2006 Participate in a seminar on “Clean room monitoring & validation” held at IBB, University of the Punjab, Lahore on 3^rd-6^th July, 2006.

2005 Training on the development of recombinant human biopharmaceuticals from International Centre of Genetic Engineering and Biotechnology (ICGBE), Italy. (Sep. – Oct. 2005).  

2004 Participate Workshop on “Quality assurance in microbiology testing laboratories to be accredited under ISO 17025” organized by UNIDO at Marroit Hotel, Islamabad on 8^th Nov. 2004.

2002 Participate SAARC workshop on “Tissue Engineering and Banking” under SAARC-Japan special fund (Bangladesh Atomic Energy Commission) from 9^th-11^th September 2002 at Dhaka, Bangladesh. 

2000 Paper presentation on “Development of commercial formulation of Bt. biopesticide” in 3^rd Biennial conference of Pakistan Society for Microbiology, 28-30 March, 2000 organized by Karachi University at PC, Lahore.

1997 International Symposium-workshop on Genomics and Computational Analysis, October 16-18, 1997. CEMB, Lahore, Pakistan.

1997 Paper presentation on “Propagation of Bacillus thuringiensis for the development of bioinsecticide” in fifth International Symposium workshop on the applications of Molecular Biology Research in Agriculture, Health and Environment, October 14-15, 1997. CEMB, Lahore, Pakistan.

1997 Work on “Development of Genetically engineered insecticide” in Dr. Wang Jinshang’s Lab. at Nanjing Agriculture University, Nanjing, China from 06.05.97 to 19.07.97 as a researcher exchange programme between Pakistan and China.

1995 Fourth International Symposium Workshop on the Applications of Molecular Biological Research in Agriculture, Health and Environment, April 9-11, 1995, CEMB, Lahore Pakistan.

1994 SAARC Training Workshop on Gene Cloning, April 29-May 12, 1994, CEMB, Lahore, Pakistan.

1994 Poster Presentation. Coordination meeting of Institutional Excellence Programme (IEP) January, 1994, Sponsored by University Grants Commission (UGC), Islamabad, Pakistan.

1992 Workshop and Regional Training Course on In vitro fertilization and Embryo Transfer in Buffalo, April 11-16, 1992, Animal Sciences Institute, NARC, Islamabad, Pakistan.

1992 Training on laparoscopic embryo transfer technology in small ruminants in Dr. B. Abdul Rashid’s laboratory at Malaysian Agriculture Research and Development Institute (MARDI), Kuala Lumpur, Malaysia, from July 1992 to October 1992.

1992 Workshop on the Application of new Technologies in Sheep/Goats Reproduction, September 15, 1992, MARDI, Keluang, Malaysia.

1991 SAARC Symposium Workshop on the Biological Control of Agriculturally Important Plant Pests, December, 16-18, 1991, National Centre of Excellence in Molecular Biology, (CEMB), Lahore, Pakistan.

1990-91 Research on “cloning and expression of fullength and truncated human estrogen and insulin receptor genes to E. coli and Baculovirus expression systems”, with Dr. Lim Tung and Dr. T.R.Butt, at the School of Medicine, University of Pennsylvania, Philadelphia, USA.

1989 A Laboratory Workshop on Identification of Cloned Gene Products, November, 4-17, 1989, CAMB, Lahore, Pakistan. Member Organizing Committee.

1988 Third Regional Training Workshop on Recombinant DNA Techniques.  December 10-31, 1988, CAMB, Lahore, Pakistan. Member Organizing Committee.

1987 Second International Symposium Workshop on the Application of Genetic Engineering, October 18-22, 1987, CAMB, Lahore, Pakistan.

1987 A training course on Microbial technologies, September, 26-October. 15, 1987, CAMB, Lahore, Pakistan. Member Organizing Committee.

1987 International Symposium on the Application of Molecular Biology, April 2, 1987, Centre for Advanced Molecular Biology, (CAMB) Lahore, Pakistan.

Journal papers

Publications (International)

1. Muhammad Ijaz¹, Gulzar Ahmad¹, Farida Anjum², Usman Zeb³, Noor Muhammad⁴, Irfan Khan⁵, Sidra Usman¹, Abrar Hussain1, Shumaila Ubaid6, Abdul Haseeb Rahim1, Umbarin Latif2, Humaira Gul², Rahim Shah7, Hafsa Shah1, Faryal Azam1, Zia Ur Rahman8, Muhammad Ayaz9, Ahmad Usman Zafar¹⁰, Faraz Ahmad Khan1, Hafiza Wajeeha Zahid1, Sulaiman Faisal1* (2023). Morphological identification and resistance profile of antibiotic and heavy metals resistant bacteria in hospital sewage of Peshawar. Advances in Life Sciences, vol.10 issue 3; pp 452-456.        (I.F.1.6)
2. Zia Ur Rahman, Nadeem Ahmed, Numan Fazal, Muhammad Islam Khan, Mohsin Ahmed khan, Saad Tahir, Muhammad Akram, Sajjad Ullah, Ahmad Usman Zafar(2023). Enhancing the Expression and Purification of IL-29: A study of autoinduction and one-step Purification Methods. Journal of life sciences Vol.10 (1) p 122-28; ISSN 2310-5380.   (IF. 6.78)  
3. Rabbia Pervaiz, Mohsin Ahmad Khan, Faiz Ahmed Raza, Sohail Ahmad, Ahmad Usman Zafar,Nadeem Ahmed, Maham Akram. (2022)  Expression of a mosquito larvicidal gene in chloroplast and nuclear compartments of Chlamydomonas reinhardtii. J Biotechnology (360) 182-191                                     (IF 3.595)
4. Fakiha Bajwa, Nadeem Ahmed, Mohsin Ahmad Khan, Farhana Azam, Muhammad Akram, Saad Tahir, AhmadUsman Zafar (2019-20). Evaluating the ion exchange chromatography for matrix‐assisted PEGylation and purification of consensus interferon. Biotechnology & Applied Biochemistry, 67(2)296-205; hptt //doi.org/10.1002/bab.1832.                                                                                                              (IF. 1.638)
5. Rabbia Pervaiz , Mohsin Ahmad Khan,  Nadeem Ahmed,  Hafsa Naeem, Ahmad Usman Zafar.(2018) Determination of mosquito Larvicidal potential of  Bacillus thuringiensis Cry11Ba fusion protein through molecular docking. Biologia, 73: 1015-1023.  org/10.2478/s11756-018-0103-9       (Aug. 2018) (IF 0.696)
6. Faidad Khan, Mohsin Ahmad Khan, Nadeem Ahmed, Islam Khan, Hamid Bashir,  Saad Tahir   & Ahmad Usman Zafar(2018). Molecular characterization of Pneumococcal surface protein A(PspA), serotype distribution and antibiotic susceptibility of  Streptococcus pneumoniae strains isolated from Pakistan. Infect Dis Ther,  7:277–289; doi.org/10.1007/s40121-018-0195-0                                             (IF 1.24)
7. Ahmed  N., Abbas R., Khan M.A., Bashir H, Tahir S, Zafar A.U.,(2017). Enhancing recombinant interleukin-6 production yield by fermentation optimization, two step denaturing and one step purification. Biotechnology and Applied Biochemistry. DOI:10.1002/bab.1588., 1-7   (IF 1.413)      
8. Khan, M. A., Ahmed, N., Khan, M. I., Zafar, A. U.,Tahir, S., Anjum, M. S., Ali, M., Khan, F., Husnain, T. (2017). Bioactivity studies of Huh-7 cells derived human epidermal growth factor expressed in Pichia pastoris. Bioscience, Biotechnology, and Biochemistry, 81(6), 1114-1119.     (IF 1.176)      
9. Bashir, H., Ahmed, N, khan, M.A., Zafar, A.U., Tahir, S., Kanwal. H., Khan. F., Rahman. Z., Husnain, T. ( 2016). Evaluating the auto induction Expression system and one step purification for high level Expression and purification of gallbladder derived rhIL-1ra. Biotechnology and Applied Biochemistry. DOI:10.1002/bab.1463, 20-26.     (IF 1.413)
10. N., Tahir S., RaA.Q., Hassan S., Khan A., Latif A., Khan M., Tabassum B., Shahid A.A., Zafar A.U.,Husnain T. (2015) Escherichia coli expression of NDV fusion protein gene and determination of its antigenic epitopes. Biologia, 70(12)  1553-1564.     DOI: 10.1515/biolog-2015-0191.      (IF 0.803)    
11. Bashir, H.; Ahmed,N, Khan,M.A., Zafar, A.U., Tahir, S., Khan,I.M., Khan, F., Husnain, T. (2015). Simple procedure applying lactose induction and one step purification for high yield production of rhCIFN. Biotechnology and Applied Biochemistry. 63(5):708-714.  DOI: 10.1002/bab.1426.    (IF 1.8)
12. Ahmed, N*., Bashir, H., Zafar, A. U., Khan, M. A., Tahir, S., Khan, F., Khan M.I., Akram M. Husnain, T. (2015) Optimization of conditions for high‐level expression and purification of human recombinant consensus interferon (rh‐cIFN) and its characterization. Biotechnology & Applied Biochemistry. 62(5), 699-708. DOI:10.1002/bab.1320    (IF 1.8)        
13. Ahmed N, Zafar AU, Khan M.A, Tahir S, Khan M.I., Bashir H, Khan F, Sarwar S, Ilyas S, Husnain T. (2014)  Matrix assisted refolding and purification of placenta derived recombinant human interleuki-6 produced in Escheria coli. Biotechnology & Applied Biochemistry. 61(5)  541-548. DOI:10.1002/bab.1205     (IF1.89)
14. Khan, M A., Khan F., Ahmad N., Khan M I., Zafar A U.,Husnain T., (2014) Expression line approach to recombinant human epidermal growth factor into yeast, Pichia pastoris from Huh-7 cell line. Molecular Biology Reports 41(3)  1445- 1451     DOI 10.1007/s 11033-013-2989-1      (IF2.5)    
15. Khan M A., Hassan N., Ahmad N., Khan M I., Zafar A U., Khan F., Husnain T., (2014).  Studies to analyze the relationship between IFNα2b gene dosage and its expression, using a Pichia pastoris-based expression system. Yeast  31(1)  13-28.  DOI:10.1002/yea.2990   (IF1.955)        
16. US Patent:Riazuddin,S., Rehman, Atta-ur., Zafar, A Usman., Choudhary,I., Ashfaq, U A., Khalid M., Husnain, T., Ali, Z., Akram, J.,  (2012) 7-nitro-2-(3-nitro phenyl)-4H-3,1-benzoxazin-4-one or derivatives thereof for treating or preventing antiviral infections.  US Patent # 8,148,368.             
17. Shagufta Naz, Shahbaz Ali, S Amer Riazuddin, Tahir Farooq, Nadeem H Butt, Ahmad U Zafar, Shaheen N Khan, Tayyab Husnain, Ian M MacDonald, Paul A Sieving, J Fielding Hejtmancik, and Sheikh Riazuddin. (2011)  Mutations in RLBP1 associated with fundus albipunctatus in consanguineous Pakistani families. British Journal of  Ophthalmology  95 (7)  1019 – 1024.  DOI:10.1136/bjo.2010.189076.    (IF 2.91)               
18. Nadeem Ahmed, Mohsin Ahmad Khan, Naila Shahid, Idrees Ahmed Nasir and Ahmad Usman Zafar.(2011) One step purification of biological active human interleukin-2 protein produced in yeast (Pichia Pastoris). African Journal of Biotechnology 10 (67)  15170-15178.   DOI: 10.5897/AJB11.2116 (IF 0.57)   
19. Mohsin Ahmad Khan, Nadeem Ahmad1, Ahmad Usman Zafar,Idrees Ahmad Nasir and Muhammad Abdul Qadir.  (2011)  Isolation and screening of alkaline protease producing bacteria and physio-chemical characterization of the enzyme. African Journal of Biotechnology 10(33)  6203-6212.  ISSN 1684-5315@ 2011 (IF 0.57).
20. Namerah Sabir,1 S. Amer Riazuddin,1,2 Tariq Butt,1 Farheena Iqbal,1 Idrees A. Nasir,1 Ahmad U. Zafar,1 Zaheeruddin A. Qazi,3 Nadeem H. Butt,4 Shaheen N. Khan,1 Tayyab Husnain,1 J. Fielding Hejtmancik,5 and Sheikh Riazuddin1,4.  (2010) Mapping of a new locus associated with autosomal recessive congenital cataract to chromosome 3q. Molecular Vision  16,    2634 – 2638.  PMCID:PMC3002966       (IF 2.511)     
21. Namerah Sabir, S. Amer Riazuddin, Haiba Kaul,1 Farheena Iqbal, Idrees A. Nasir, Ahmad U. Zafar, Zaheeruddin A. Qazi, Nadeem H. Butt, Shaheen N. Khan, Tayyab Husnain, J. Fielding Hejtmancik, and Sheikh Riazuddin. (2010)  Mapping of a novel locus associated with autosomal recessive congenital cataract to chromosome 8p. Molecular Vision  16,  2911–2915.  PMID:PMC3013063     (IF 2.511)  
22. Riazuddin SA, Shahzadi A, Zeitz C, Ahmed ZM, Ayyagari R, Chavali VR, Ponferrada VG, Audo I, Michiels C, Lancelot ME, Nasir IA, Zafar AU, Khan SN, Husnain T, Jiao X, MacDonald IM, Riazuddin S, Sieving PA, Katsanis N, Hejtmancik JF. (2010) A mutation in SLC24A1implicated in autosomal-recessive congenital stationary night blindness.  American Journal of Human Genetics    87(4)  523 – 531.  PMCID: PMC2948789. DOI:10.1016/j.ajhg.2010.08.013  (IF 11.716)     
23. Haiba Kaul; S. Amer Riazuddin; Zaheeruddin A. Qazi; Idrees A. Nasir; Ahmad U. Zafar;Shaheen N. Khan; Tayyab Husnain; Javed Akram; J. Fielding Hejtmancik; Sheikh Riazuddin. (2010)  Ectopia Lentis in a Consanguineous Pakistani Family and a Novel Locus on Chromosome 8q. Archives Ophthalmology vol.128(8)  1046-1049  PMID:20697006         (IF 3.2)           
24. Yasmeen A, Riazuddin SA, Kaul H, Mohsin S, Khan M, Qazi ZA, Nasir IA, Zafar AU,Khan SN, Husnain T, Akram J, Hejtmancik JF, Riazuddin S. (2010) Autosomal recessive congenital cataract in consanguineous Pakistani families is associated with mutations in GALK1.  Molecular Vision  16,  682 – 688    PMCID:PMC2855732            (IF 2.511)         
25. Haiba Kaul, S. Amer Riazuddin, Mariam Shahid, Samra Kousar, Nadeem H. Butt, Ahmad U. Zafar, Shaheen N. Khan, Tayyab Husnain, Javed Akram, J. Fielding Hejtmancik, and Sheikh Riazuddin. (2010) Autosomal recessive congenital cataract linked to EPHA2 in a consanguineous Pakistani family. Molecular Vision  16,   511 – 517.     PMCID:PMC2846848      (IF 2.511)    
26. Khan SY, Riazuddin S, Shahzad M, Ahmed N, Zafar AU,Rehman AU, Morell RJ, Griffith AJ, Ahmed ZM, Riazuddin S, Friedman TB. (2010) DFNB79: reincarnation of a nonsyndromic deafness locus on chromosome 9q34.3. Eur J Human Genetics    18(1)   125 – 129        doi:10.1038/ejhg.2009.121.    (IF 4.4)  
27. Riazuddin S, Anwar S, Fischer M, Ahmed ZM, Khan SY, Janssen AG, Zafar AU,Scholl U, Husnain T, Belyantseva IA, Friedman PL, Riazuddin S, Friedman TB, Fahlke C. (2009) Molecular basis of DFNB73: mutations of BSND can cause nonsyndromic deafness or Bartter syndrome.  American  J Human Genetics 85(2)  273 – 280. PMCID:PMC2725234    (IF 12.303)          
28. Waryah AM, Rehman A, Ahmed ZM, Bashir ZH, Khan SY, Zafar AU, Riazuddin S, Friedman TB, Riazuddin S.(2009) DFNB74, a novel autosomal recessive nonsyndromic hearing impairment locus on chromosome 12q14.2-q15. Clinical Genetics  76(3)   270 – 275       PMID:19650862  doi:10.1111/j.1399-0004-2009.01209.x     (IF 3.304)       
29. Saima Riazuddin , Sabiha Nazli , Zubair M. Ahmed , Yi Yang , Fareeha Zulfiqar , Rehan S. Shaikh , Ahmed U. Zafar, Shaheen N. Khan , Farooq Sabar , Fouzia T. Javid , Edward R. Wilcox , Ekaterini Tsilou , Erich T. Boger , James R. Sellers, Inna A. Belyantseva , Sheikh Riazuddin , Thomas B. Friedman. (2008)   Mutation spectrum of MYO7A and evaluation of a novel nonsyndromic deafness DFNB2 allele with residual function. Human Mutation  29 (4)  502 – 511.    PMID:18181211    DOI:10.1002/humu.20677      (IF 7.033)       
30. Ain1, S. Nazli, S. Riazuddin, A.Jaleel, S. A. Riazuddin, A.U.Zafar, S. N. Khan, T. Husnain1, A. J. Griffith, Z. M. Ahmed, T. B. Friedman and S. Riazuddin. (2007)  The autosomal recessive nonsyndromic deafness locus DFNB72 is located on chromosome 19p13.3 .  Human Genetics  122 (5),   445 – 450    PMID:17690910.   DOI:10.1007/s00439-007-0418-z        (IF 3.97)    
31. Kitajiri S-i, McNamara R, Makishima T, Husnain T, Zafar AU, Kittles RA, Ahmed ZM, Friedman TB, Riazuddin S, Griffith AJ. (2007) Identities, frequencies and origins of TMC1mutations causing DFNB7/B11 deafness in Pakistan. Clinical Genetics. 72 (6)  546 – 550.    PMID:17877751.  doi:10.1111/j.1399-0004.2007.00895.x   (IF 3.18)  
32. Riazuddin S, Khan SN, Ahmed ZM, Ghosh M, Caution K, Nazli S, Kabra M, Zafar AU,Chen K, Naz S, Antonellis A, Pavan WJ, Green ED, Wilcox ER, Friedman PL, Morell RJ, Riazuddin S, Friedman TB. (2006) Mutations in TRIOBP, which encodes a putative cytoskelatal-organizing protein, are associated with nonsyndromic recessive deafness. American Journal of Human Genetics  78 (1), 137-143 ;   PMCID:PMC1380211. (IF 12.629)           
33. Shahid A.A., I.A.Nasir, U.Zafar, B. Chaudry and S.Riazuddin. (2004) The Camb biopesticides: Preparation, uses and comparative studies with chemical pesticides. Review In: Biotechnology in Environment Management. Chapter 12, ABD Publisher, Jaipur, India. ed. 1st, p260-282.

Publications (National)

1. Sabar,M.F., Kausar,S., Zafar A.U,Shahid M. (2013) PEG-interferon conjugates: Effects of length and structure of linker. Pak.J.Pharm.Sci., 26(2), 425-430.   
2. Zafar A.U.,and S.Riazuddin. (2009) A method of purification of the recombinant protein products. Patent # 140587
3. Zafar A.U.,and S.Riazuddin. (2009) A process for modification of recombinant human interferon for therapeutic use. Patent # 140586
4. Zafar A.U.,and S.Riazuddin. (2007) An improved codon optimized human interferon. Pakistan patent office, Karachi. Patent #  140574.
5. Zafar A.U.,A.Qadeer and S.Riazuddin. (2003) Scale up production of spore/crystal protein by Bacillus thuringiensis in the stirred fermentor. Pak. J.  Sci. & Indus. Res  46(4), 242-245.
6. Shahid,A.A., I.A.Nasir, Zafar A.U, A.Sumrin, B.Chaudhry and S.Riazuddin, (2003)  The use of CAMB biopesticides to control pests of rice (Oryza sativa). Asian J.Plant Sci  2(15-16), 1079-1082.
7. Zafar, A.U.& S. Riazuddin. (2002) Propagation of locally isolated Bacillus thuringiensis (CAMB-3023) for the production of spore-crystal protein in shake flask. Pak. J. Sci. Ind. Res   45(6), 400-403.
8. Zafar, A.U.,A.Nasir, A.A.Shahid, M.S.Rahi and S.Riazuddin. (2002) Performance evaluation of CAMB biopesticides to control cabbage butterfly (pieris brassicae) in cauliflower crop.  Pak. J. Biol. Sci  5(10), 1041-1043.
9. Rehman, M.Z., U. Zafar, I.A .Nasir,  M.A. Qadeer and S. Riazuddin. (2002) Comparative study of Bacillus thuringiensis biopesticide against cotton bollworm. Asian J.Plant Sci., 1(5), 574-576.
10. Karim, A.U. Zafar, I.A.Nasir & S.Riazuddin. (2000) Field efficacy of CAMB Bacillus thuringiensis biopesticide to control Helicoverpa armigera (Hübnar) and Earias vitella (Fabricius) in Okra crop. Pak. J. Biol. Sci.  3 (8), 1296-1298.
11. Zafar A.U.& S. Karim, A. Nasir & S. Riazuddin. (2000) Shelf life and field evaluation of CAMB Bacillus thuringiensis biopesticide against Heliocoverpa Armigera (Hübnar) (Lepidoptera: Noctuidae) on Tomato. Pak. J. Biol. Sci   3 (5), 804-807.
12. Zafar A U, Qadeer M A, Riazuddin S. (2000) Process development for Bacterial biopesticides. Pakistan patent office, Karachi. Patent # 137087.
13. Khan, S.N., Zafar, A.U., Riazuddin, S. (1995) Molecular genetic diagnosis of β-thalassemia. JPMA, Pakistan 45(3)  PMID:7596015, 66-70.

Patents

1. Riazuddin; Shaikh (Lahore, PK), Rehman; Atta-ur (Karachi, PK), Zafar; Usman   (Lahore, PK),Choudhary; Iqbal (Karachi, PK), Ashfaq; Usman Ali (Lahore, PK), Khan; Khalid M. (Lahore, PK), Husnain; Tayyab (Lahore, PK), Khan; Zulfiqar Ali (Lahore, PK), Akram; Javed. (2012) 7-nitro-2-(3-nitro phenyl)-4H-3,1-benzoxazin-4-one or derivatives thereof for treating or preventing antiviral infections. US Patent, USA  # 8,148,368.

2. Zafar A.U.and S. Riazuddin (2009) A method of purification of the recombinant protein products. Pakistan patent office, Karachi #140587

3. Zafar A.U.and S. Riazuddin (2009) A process for modification of recombinant human interferon for therapeutic use. Pakistan patent office, Karachi #140586

4. Zafar A.U.and S. Riazuddin (2007) An improved codon optimized human interferon. Pakistan patent office, Karachi #140574

5. Zafar, A.U., A. Qadeer and S. Riazuddin (2000) Process development for Bacterial biopesticides. Pakistan patent office, Karachi #137087

Professional Achievements

• Establish & organize a functional Biotech lab at Qarshi University.
• Training / experience on cGMP compliance for recombinant therapeutic proteins and stem cell production facility.
• SOP’s documentation, implementation and training for cGMP compliances. ISO 17025 for CEMB Biopharmaceutical QC labs.
• Established infrastructure for pilot scale fermentation and commercial production of recombinant human proteins for therapeutic use.
• Established mammalian cell culture lab and providing national facility for quality control testing of recombinant human proteins to NIH, Islamabad and private pharma sector.
• Established and patented 3 patents for the downstream process of recombinant human interferon for therapeutic use.
• Established and Patented downstream process for the commercial production of Bt. based biopesticide against lepidoptera.
• Prepared three different formulations of diptericidal Bt. based biopesticides.
• Developed Probiotic formulation for poultry.
• Established CEMB mammalian cell repository and bacterial culture collection repository of 2000 isolates.
• Established animal house facility for the stem cell project.